Expression changes of miR-17-5 p in cervical cancer tissues and its effects on cervical cancer cell growth and proliferation
WEI Qian
ZHANG Peng
Abstract：Objective To observe the expression changes of miR-17-5p in the cervical cancer tissues and its effects on cervical cancer cell growth and proliferation. Methods The levels of miR-17-5p in cervical cancer tissues and adjacent tissues of 20 patients were detected by real-time quantitative PCR. We divided the human cervical cancer cell line HeLa in-to different groups, which were respectively transfected by artificial miR-17-5p plasmid, negative control plasmid, miR-17-5p antisense oligonucleotide (ASO) plasmid and irrelevant sequence oligonucleotide plasmid. MTT and colony experiment were used to detect the growth activity and clone number. Results The relative expression of miR-17-5p in the cervical cancer tissues and adjacent tissues were 0. 315 ± 0. 185 and 1. 143 ± 1. 373 (P<0. 05). The cell growth and cloning effi-ciency of HeLa cells transfected with pri-miR-17-5p were significantly decreased as compared with those of HeLa cells transfected by negative control plasmid (all P<0. 05);the cell growth and cloning efficiency of HeLa cells transfected with pri-miR-17-5p ASO were significantly increased as compared with those of HeLa cells transfected by irrelevant sequence oli-gonucleotide plasmid (all P<0. 05). Conclusion The expression of miR-17-5p was significantly down-regulated in the cervical cancer tissues, which inhibited the cell growth and proliferation of cervical cancer cells.
Keywords：polycistronic mRNAmiR-17-5p genecervical neoplasmscervical carcinomacervical carcinoma cellscell growthcell proliferation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 8-10 )