Influence of Baihu decoction on lung injury of rats with systemic inflammatory response syndrome

ZHENG Xing-zhen; YU Qiang; ZHENG Guo-xiang

doi:10.3969/j.issn.1002-266X.2015.42.007

Abstract：Objective To observe the effect of Baihu decoction on the changes of TNF-ɑand lung tissues of rats with systemic inflammatory response syndrome ( SIRS) , and to investigate the influence and mechanism of Baihu decoction on the lung tissues of the SIRS model rats .Methods Sixty rats were randomly divided into the control group , model group , and Chinese medicine Baihu decoction treatment group ( Chinese medicine group ) and Western medicine hydrocortisone so-dium succinate treatment group ( Western medicine group ) , each group had fifteen rats .The SIRS model rats were made in the model group, Chinese medicine and Western medicine group by escherichia coli lipopolysaccharide (20 mg/kg).After modeling , the rats of the model group , Western medicine group and the Chinese medicine group were respectively treated with normal saline by gavage, hydrocortisone sodium succinate and Baihu decoction by vein for 3 days.On the fourth day, the rat abdominal aortic blood of each group was acquired , and the plasma TNF-ɑlevel was measured by enzyme-linked im-munosorbent assay (ELISA), then we killed the rats and took out the lung tissues .The pathological changes of the lung tissues were observed by the naked eyes and light microscopy after HE staining .Results The levels of TNF-ɑof the con-trol group, model group, and Chinese medicine group and Western medicine group were respectively (64.05 ±7.72), (74.25 ±8.67), (62.63 ±7.01) and (58.78 ±9.68) pg/mL;the TNF-ɑlevel of the model group was higher than that of the control group (P<0.05), the TNF-ɑlevels of the Chinese medicine group and Western medicine group were lower than that of the model group (P<0.05).Under the light microscopy, the lung tissues of the control group had a structural integrity, clear alveolar space and alveolar septa without edema , no inflammatory cell infiltration and other changes; the model group had interstitial pulmonary congestion complicated with inflammatory cell infiltration , and thickened alveolar septa.The histopathological changes of the Chinese medicine and Western medicine group were significantly lighter than that of the control group , and the histopathological change of the Chinese medicine group was better than that of the Western medicine group .Conclusion Baihu decoction has protective effect on the lung tissues of SIRS rats , and the mechanism may be related to decrease of TNF-αrelease.