Effect of WJ-MSCs transplantation on expression of CD4+CD2+5 Foxp3+Treg cells in peripheral blood of rats with heart failure
ZHAO Zi-lin
YE Chang-qing
LUO Jian-chun
MA Li-xian
MA Wu-zhi
LIANG Xiao-hui
HUANG Liang-yan
Abstract：Objective To observe the levels of N-terminal pro-brain natriuretic peptide ( NT-proBNP) and the per-centage change of CD4+CD2+5 Foxp3+Treg cells in rats with heart failure ( HF) and to evaluate the effect of Wharton's jelly mesenchymal stem cells ( WJ-MSCs) transplantation on the immune activation of HF rats.Methods Forty-eight male Wistar rats were selected and 16 rats were fed normally without any treatment which was taken as the blank group, the rest of the rats were subcutaneously injected with isoprenaline to make the HF models, after the success of modeling, they were randomly divided into the stem cell transplantation group (n=16) and the model group (n=16).The rats in the stem cell transplantation group was administered with intramyocardial injection of WJ-MSCs suspension, while the rats in the model group was injected with the same amount of normal saline.Four weeks later, CD4+CD2+5 Foxp3+Treg cell proportion of the peripheral blood was detected by flow cytometry, the levels of NT-proBNP, TNF-αand IL-10 in the serum of the three groups were detected by enzyme-linked immunosorbent assay ( ELISA) , and the heart tissues of rats were collected for the immunohistochemical staining.Results The levels of NT-proBNP and TNF-αin the plasma of HF rats were significantly higher than those of the blank group, the CD4+CD2+5 Foxp3+Treg cell proportion in the peripheral blood and the level of IL-10 was significantly lower than that of the blank group (P<0.05).Four weeks after cell transplantation, compared with the model group, NT-proBNP and TNF-αwere significantly decreased, while the CD4+CD2+5 Foxp3+Treg cell proportion in the peripheral blood and the IL-10 level were significantly increased in the stem cell transplantation group, and the differ-ence was statistically significant (all P<0.05).There was negative correlation between the level of CD4+CD2+5 Foxp3+Treg cell and NT-proBNP expression (r=-0.634,P<0.05).Conclusion WJ-MSCs transplantation can significantly in-crease the CD4+CD2+5 Foxp3+Treg cell proportion in the peripheral blood of HF rats, inhibit inflammatory reaction and im-prove the symptoms of HF.
Keywords：heart failureumbilical cord mesenchymal stem cellsCD4+CD2+5 Foxp3+Treg cellsrats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 13-15 )