Effect of sevoflurane preconditioning on serum cTnI and Nrf2 expression in peripheral blood mononuclear cells of patients undergoing cardiac valve replacement
SUN Xue-mei
WANG Rui-ting
HE Ke-qiang
ZHANG Wei
CHAI Xiao-qing
PAN Jian-hui
Abstract：Objective To observe the effect of sevoflurane preconditioning on serum cTnI and the nuclear factor ery-throid 2-related factor 2( Nrf2) expression in peripheral blood mononuclear cells ( PBMCs) of patients undergoing cardiac valve replacement with CPB, and to investigate its influence on the myocardial protection and oxidative stress of body. Methods In this randomized controlled trial, thirty patients who received valve replacement were randomly divided into two groups (n=15): the control group (group C) and sevoflurane preconditioning group (group S).In the group S, 1.0%sevoflurane was inhaled for 20 min from skin incision, and followed with oxygen elution for 10 min, it repeated twice.The artery blood samples were collected for the measurements of serum concentrations of cTnI, MDA and SOD im-mediately before anesthesia induction ( T1 ) , at 2 h ( T2 ) , 24 h ( T3 ) and 48 h ( T4 ) after aorta declamping respectively. The expression of Nrf2 protein from PBMC was detected by Western blotting at T1 and T3 .Linear correlation between the expression of Nrf2 protein and MDA concentration, SOD activity was analyzed.Results Compared with that before opera-tion, the concentration of serum cTnI was increased significantly in both groups(P<0.05) at T2-4.However, it was much lower in the group S than in the group C(P<0.05).The expression of Nrf2 protein in PBMC was increased significantly in both groups at T3 as compared with that before operation (P<0.05).However, it was much higher in the group S than in the group C (P<0.05).Compared with group C, the serum MDA concentration was decreased, while SOD activity was significantly higher at T2-4 in the group S (P<0.05).There was linear correlation between the expression of Nrf2 protein and MDA concentration, SOD activity (r=-0.694 and 0.739, all P<0.05).Conclusion Sevoflurane preconditioning can alleviate myocardial injury and reduce oxidative stress injury in patients undergoing cardiac valve replacement with CPB, which might be associated with the increased Nrf2 expression.
Keywords：sevofluranecardiopulmonary bypassNF-E2-related factor 2cardiac troponin Ioxidative stress
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 4-6 )