Protective effect of imatinib mesylate on hyperoxia-exposed lung injury in newborn rats and its mechanism

SHA Ning; TIAN Zhao-fang; LI Yu-hong

doi:10.3969/j.issn.1002-266X.2015.40.007

Abstract：Objective To observe the protective effect of imatinib mesylate on hyperoxia-exposed lung injury in new-born rats and to investigate its mechanism.Methods Twenty-four newborn Sprague-Dawley rats were randomly divided in-to three groups, 8 in each group:the air-exposed group ( group A) , hyperoxia-exposed group ( group B) and hyperoxia-ex-posed +imatinib mesylate group ( group C) .The rats in the group C were intraperitoneally injected with 50 mg/( kg? d) imatinib mesylate for 7 days, while rats in the groups A and B were injected with the same amount of normal saline for 7 days.Then all the rats were exposed to air, and were sacrificed 10 days later.The levels of platelet-derived growth factor-B ( PDGF-B) in bronchoalveolar lavage fluid ( BALF) and in serum were detected by ELISA, the expression of PDGF-B mRNA in tissue homogenates were detected by RT-PCR, and the value of lung damage score was calculated after He stai-ning under light microscope.Results There were no significant differences in the levels of PDGF-B in serum among three groups (all P>0.05).The levels of PDGF-B in BALF of groups A, B and C were (66.15 ±3.99), (103.91 ±6.79) and (85.08 ±6.33) pg/mL, and there was significant differences among these three groups (all P<0.05).The expres-sion of PDGF-B mRNA in the lung tissue homogenates was 0.26 ±0.04, 0.44 ±0.09 and 0.32 ±0.03, and there was sig-nificant difference among these three groups (all P<0.05).The lung damage score in the groups A, B and C was (0.62 ±0.21), (2.32 ±0.38) and (1.61 ±0.46) scores, and there were significant differences among these three groups (all P<0.05).Conclusion Imatinib mesylate protects hyperoxia-induced lung injury through PDGF and platelet-derived growth factor receptor.