Effect of methanol extract from lasianthus lancilimbus on proliferation of human esophagus carcinoma cells EC-9706

WEN Yang-min; WAN Duan-jing; XIE Yong-hua; LUO Cai-lin; QIU Dan-ying

doi:10.3969/j.issn.1002-266X.2015.40.004

Abstract：Objective To investigate the inhibitory effect of methanol extract from lasianthus lancilimbus ( MESLL) on proliferation of human esophagus carcinoma cells EC-9706 and the mechanism.Methods EC-9706 cells were cultured in vitro.①Observing the effect of MESLL on cell proliferation:the cells in the logarithmic growth phase were divided into MESLL group, cyclophosphamide ( CTX) group and control group.MESLL group was respectively treated with 0.01, 0.05, 0.1, 0.2 and 0.4 mg/mL MESLL, the CTX group was intervened by 1 mg/mL CTX and the control group was not treated.The proliferation inhibiting rates were detected after 24, 48 and 72 h.②Observing the effect of MESLL on NO ac-tivity and the levels of NOS and cGMP:the cells in the logarithmic growth phase were divided into the experimental group and the control group.The experimental group was respectively treated with 0.01, 0.05, 0.1, 0.2 and 0.4 mg/mL MESLL for 48 h, and the control group was not treated.The NO activity and the levels of NOS and cGMP were measured. Results ①After 24 h, the proliferation inhibiting rates treated with 0.2 mg/mL and 0.4 mg/mL MESLL were higher than those of the CTX group (all P<0.05).After 48 and 72 h, tumor inhibiting rate of MESLL group was increased with the increased concentrations of MESLL.The tumor inhibiting rates treated with 0.1 mg/mL, 0.2 mg/mL and 0.4 mg/mL MESLL were higher than those of the CTX group (all P<0.05).②Effect of MESLL on NO activity and the levels of NOS and cGMP:the activity of NO, the levels of NOS and cGMP reduced with the increased concentrations of MESLL ( P<0.05).Conclusions MESLL could inhibit the proliferation of EC-9706 cells, and the mechanism may be related with the inhibition of NO/NOS-cGMP pathway.