Intervention of sevoflurane pretreatment on rat pulmonary injury caused by liver ischemia reperfusion and its mechanism

WANG Xiao-li; XU Gui-ping; GUAN Xiao-meng

doi:10.3969/j.issn.1002-266X.2015.39.006

Abstract：Objective To observe the intervention of sevoflurane pretreatment on rat pulmonary injury caused by liver ischemia reperfusion and to explore its mechanism .Methods A total of 30 healthy Wistar rats were randomly divided into sham-operation group , the model group and the sevoflurane group , with 10 rats in each group .Models of liver ischemia reperfusion were established in the model group and sevoflurane group , and rats of the sevoflurane group were given inhala-tion of sevoflurane for 30 min before modeling; while in the sham-operation group , only porta hepatis was separated but blood supply was not blocked .Rats were scarified at 60 min after reperfusion , and the serum alanine aminotransferase ( ALT) , aspertate aminotransferase ( AST) and tumor necrosis factor-α( TNF-α) were detected; part of right upper lobe was used to measure the wet/dry weight ratio ( W/D ratio) and part was used to make paraffin section for pathological ob-servation;and left lung tissue was taken to determine malondialdehyde ( MDA) and MMP-9 mRNA.Results Lung tissue of the sham-operation group was normal in structure under microscopy , while damage degree of lung tissue in the sevoflu-rane group was reduced as compared with that of the model group .Serum ALT, AST and TNF-αof the model group and sevoflurane group were increased as compared with those of the shan-operation group (all P<0.05); while serum ALT, AST and TNF-αof the sevoflurane group was lower than that of the model group (all P<0.05).W/D ratio, MDA content and relative expression of MMP-9 mRNA in the lung tissues of the model group and sevoflurane group were increased as compared with those of the sham-operation group ( all P<0.05 ); W/D ratio, MDA content and relative expression of MMP-9 mRNA in the lung tissues of the sevoflurane group were lower than those of the model group (all P<0.05).Con-clusion Sevoflurane can alleviate the pulmonary injury caused by liver ischemia reperfusion , whose mechanism may be re-lated to the lower levels of TNF-αand inhibition of MMP-9 mRNA expression, etc.