Protective effect of azelnidipine on sirolimus eluting stent-induced vascular endothelial injury and its mechanism
YUAN Ling
NIE Wei
GAO Ping
LI Xin
LIU Wei-wei
CUI Xiao-xue
Abstract：Objective To investigate the protective effect of calcium-channel blockers azelnidipine on sirolimus elu-ting stent-induced vascular endothelial injury and its mechanism .Methods Human umbilical vein endothelial cells ( HU-VECs) were cultivated and divided into the control group (treated with culture medium), model group (treated with siroli-mus 500 nmol/L) and the experimental group (azelnidipine 10 μmol/L +sirolimus 500 nmol/L), respectively.After 24 h of treatment, changes in cell morphology were observed by HE staining .Effects on the production of nitric oxide (NO) were detected by Nitric Oxide Assay Kit .The intracellular calcium ion ( Ca2+) concentration was assayed with Fluo-3/AM staining, the changes of mitochondrial membrane potential was detected by JC -1 fluorescence labeling , and the apoptosis rate of HUVECs was analyzed by Annexin V FITC/PI staining.Results After 24 h of treatment, in the control group, cells were polygonal and in the single cobblestone arrangement;in the model group, cell swelled, cytoplasm had vacuoles, part of the nucleus had pycnosis , and nuclear fragmentation were observed;in the experimental group , cells were substan-tially polygonal , and in the single cobblestone arrangement , the cell swelling and cytoplasmic vacuoles were rare .Com-pared with the control group , the levels of NO in the cell culture fluid were reduced , the levels of intracellular free Ca 2+were increased , apoptosis rate was increased and mitochondrial membrane potential was reduced in the model group and ex -perimental group (all P<0.05).Compared with the model group, the levels of NO in the cell culture fluid were in-creased , the levels of intracellular free Ca 2+were reduced , the apoptosis rate was reduced and the mitochondrial membrane potential was increased in the experimental group (all P<0.05).Conclusions Azelnidipine has protective effect on sirolimus eluting stent-induced vascular endothelial injury .The possible mechanism might be related to the decrease of in-tracellular Ca 2+which could alleviate calcium overload and mitochondrial membrane potential and finally reduce apoptosis .
Keywords：calcium-channel blockersazelnidipinedrug eluting stenthuman umbilical vein endothelial cellsvas-cular endothelial injury
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 7-9 )