Expression changes of HES1 mRNA in primary colorectal diffuse large B-cell lymphoma and its relationship with the prognosis

HU Dong-zhi; TIAN Chen; HU Jun; LIU Jian-zhong

doi:10.3969/j.issn.1002-266X.2015.39.002

Abstract：Objective To observe the expression changes of HES1 mRNA in primary colorectal diffuse large B-cell lympho-ma (DLBCL) and its relationship with the prognosis.Methods The samples of 60 newly diagnosed primary colorectal DLBCL pa-tients were collected with Ficoll after approval of the ethics committee and informed consent .Lase microdissection combined with re-al-time PCR was used to test the mRNA expression of HES1 in the tumor tissues and adjacent tissues of 60 patients.According to the results, the patients were divided into HES1-high group and HES1-low group.The overall survival (OS) and relapse-free survival (RFS) of the tow groups were compared.The Univariate analysis and Multivariate Cox regression analysis were used to analyze the related factors for tumor prognosis.Results In 60 cases of patients, the expression level of HES1 mRNA was 0.6 ±0.08 in the tumor tissues and 1.2 ±0.10 in the adjacent normal tissues, significant difference was found between them (P<0.05).The results showed that the low-expression group had a shorter OS and shorter RFS as compared with those of the high-expression group (38.6 ±1.3 vs. 53.0 ±1.4 months, 20.6 ±1.8 months vs.28.8 ±1.2 months, respectively, all P<0.05).Univariate analysis showed that age, IPI score, the diameter of tumor, the breakthrough of serous layer, LDH and HES1 mRNA were the related factors for prognosis of color-ectal primary DLBCL.Multivariate Cox regression analysis showed that the low mRNA expression of HES1 had positive implications for OS and RFS (HR=1.134, 1.134, all P<0.01).Conclusion The expression of HES1 mRNA in the primary colorectal DLBCL was decreased, and the low expression was related with the poor prognosis .