Effect of silence of S100A4 gene on apoptosis and invasion of nasopharyngeal carcinoma cell line CNE2
LUO Hai-lin
JIANG Qing-shan
SHEN Bao-ming
YANG Xue-min
Abstract：Objective To observe the effect of silencing calcium ion associated protein ( S100 A4 ) expression on ap-optosis and invasion of nasopharyngeal carcinoma cell line CNE 2.Methods The CNE2 cells were cultured , and then were divided into the blank group , control group and experimental group .The cells of the experimental group were trans-fected with S100A4siRNA by Lipofectamine2000, the control group was transfected with CON (empty plasmid) by Lipo-fectamine2000 , and the cells in the blank group did not receive special transfection .Western blotting was used to detect the S100A4 protein in CNE2 cells, and the expression of S100A4 mRNA was detected by real-time PCR.The apoptosis was detected by flow cytometry .Transwell assay was used to detect cell invasion .Results The relative expression levels of S100A4 mRNA in the experimental group, blank control group and control group were respectively 0.600 4 ±0.05, 1.000 0 ±0.00 and 0.894 1 ±0.09, and the relative expression levels of S100A4 protein were respectively 0.22 ±0.016, 0.42 ±0.022 and 0.39 ±0.022.The expression of S100A4 mRNA and protein in the experimental group was lower than that in the control group and the blank control group (all P<0.05).The apoptosis rates of the experimental group , blank group and control group were 45.87%, 3.49%and 2.49%.The apoptosis rates in the experimental group and the blank group were lower than that in the control group (all P<0.05).Transwell experimental results show that the number of cells passing through the cell membrane was (206 ±22), the blank group and the control group were (329 ±12) and (347 ±21).Significant difference was found in the number of cells between the experimental group and the blank group , the control group (all P<0.05).Conclusion After silencing S100A4 gene expression, the apoptosis of CNE2 cells was in-creased, and cell invasion ability was decreased .
Keywords：nasopharyngeal neoplasmsnasopharyngeal carcinomacalcium ion associated proteinS100 A4 proteinapoptosiscell invasion
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )