Totipotency analysis of human embryonic stem cells supported by human postnatal foreskin fibroblasts in mice
MI Qiang
HE Zhi-xu
Abstract：Objective To observe the totipotency analysis of human embryonic stem cells ( hESCs) supported by hu-man postnatal foreskin fibroblasts (hPFFs).Methods The hESCs of the 20th passage supported by hPFF and hPFF were respectively injected into the hind legs of the severe combined immunode? cient mice ( SCID) and taken as the observation and control group .After 5-week, we observe the general state and teratoma formation of the two mice .The teratoma was taken from the observation group for the histological analysis to investigate whether there were different kinds of differentia -ted cells derived from three germ layers .Results We touched the masses in the vaccination site of SCID mice on the fifth day after inoculation , and the masses increased progressively .Five weeks later , we killed the mice to strip the tumor , and then they received the histologic examination .It showed that the mass contained three germ layers'structures with the histo-logic characteristics of teratoma .Conclusions The hESCs supported by hPFFs have totipotency .
Keywords：embryonic stem cellsfibroblastsfeeder layerhumanteratomamice
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 24-25,26 )