Effects of sodium phenylbutyrate combined with gefitinib on proliferation and migration of lung adenocarcinoma A549 cells
ZHANG Zhen-an
ZHANG Jing
ZHANG Feng-lin
YUAN Ya-jun
Abstract：Objective To study the effects of sodium phenylbutyrate combined with gefitinib on proliferation and mi -gration of lung adenocarcinoma A 549 cells and to investigate the mechanism .Methods The lung adenocarcinoma A 549 cells were cultured and divided into the blank control group ( treated with DMEM medium without FBS ) , gefitinib group (treated with 1 mmol/L gefitinib), sodium phenylbutyrate group (treated with 5 μmol/L sodium phenylbutyrate ) and the combined treatment group ( treated with 1 mmol/L gefitinib and 5 mol/L sodium phenylbutyrate ) .After culture for 24 h, the OD450 was detected by MTT method and the cell cycle distribution was detected by flow cytometry .The expression of ap-optosis-related genes survivin , BRAF, Notch1, MMP-1, MMP-2, MMP-3 and MMP-9 was detected by RT-PCR.The cell migration was assayed by scratch test .Results Compared with the blank control group , the OD450 , cell proportion in S phase, cell mobility and the expression of survivin , BRAF, Notch1, MMP-1, MMP-2, MMP-3 and MMP-9 mRNA were decreased, and the cell proportion in G0/G1 phase was increased in the combined treatment group , sodium phenylbutyrate group and gefitinib group (all P<0.05).Compared with the sodium phenylbutyrate group and gefitinib group , the OD450, cell proportion in S phase , cell mobility and the expression of survivin , BRAF, Notch1, MMP-1, MMP-2, MMP-3 and MMP-9 mRNA were decreased , and the cell proportion in G 0/G1 phase was increased in the combined treatment group ( all P<0.05).Conclusion The sodium phenylbutyrate and gefitinib can both inhibit the proliferation and migration process of lung adenocarcinoma , which may be related with the decreased expression of survivin , BRAF, Notch1, MMP-1, MMP-2, MMP-3 and MMP9 mRNA.The combined application of the two drugs has synergistic effect .
Keywords：pulmonary adenocarcinomasodium phenylbutyrategefitinibcell proliferationcell migrationapopto-sisapoptosis-related genes
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 14-17 )