Effects of 17-AAG combined with cisplatin on proliferation and apoptosis of human hepatoma cell line HepG2
ZHANG Jian-qing
CHEN Mei-ni
GUO Wei
ZHAO Ju-mei
Wang Ai-hong
PANG Qiu-xia
Abstract：Objective To explore the effects of 17-AAG combined with cisplatin ( DDP) on the proliferation of human hepatocellular carcinoma HepG 2 cells and its mechanism .Methods HepG2 cells in the logarithmic phase were divided into the control group and groups A , B and C.The control group was only added with medium .Group A was added with 0.625 μmol/L 17-AAG, group B was added with 1.0 μg/mL DDP, and group C was added with 0.625 μg/mL 17-AAG and 1.0 μg/mL DDP.After being cultured for 24 h and 48 h, the cell proliferation inhibition rates of groups A , B and C were determined by MTT , the apoptosis rate was measured by flow cytometry , and the Bax and Bcl-2 mRNA in each group was measured by RT-PCR.Results The cell proliferation inhibition rates of groups A , B and C which were cultured for 48 h were all higher than those of being cultured for 24 h (all P<0.05), the cell proliferation inhibition rate of group C was higher than those of groups A and B at the same time of culture (all P<0.05).After being cultured for 48 h, the apopto-sis rate and Bax mRNA in the groups A , B and C were higher than that of the control group , while Bcl-2 mRNA was lower than that of the control group (all P<0.05); the apoptosis rate and Bax mRNA of group C were higher than those of groupsAandB,buttheBcl-2mRNAwaslowerthanthoseofgroupsAandB(allP<0.05).Conclusions 17-AAGin-hibits the growth of human hepatocellular carcinoma HepG 2 cells and has synergistic effect with cisplatin .The mechanism may be related to the up-regulation of Bax mRNA and down-regulation of Bcl-2 mRNA expression .
Keywords：17-AAGheat-shock proteins inhibitorcisplatinhepatocellular carcinomacell proliferationapopto-sisBax proteinBcl-2 protein
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 7-9 )