Effect of simvastatin on cerebral edema and TLR4、IL-6 in rats brain with intracerebral hemorrhage

ZHANG Wen-qi; ZHANG Chao; TIAN Zeng-you; ZHANG Jie-cong; LIU Zhi-jie; ZHOU Hong-xia

doi:10.3969/j.issn.1002-266X.2015.38.002

Abstract：Objective To observe the changes in the expression of TLR 4 and IL-6 in the rats after cerebral hemor-rhage,aswellastheinterventioneffectofsimvastatin.Method 60maleratswererandomlydividedintoshamgroup, model group and intervention group .To reproduce the model of cerebral hemorrhage , we injected the fresh autologous blood into the right side of the brain basal ganglia in the rats .The method of Garcia was used to evaluate the score of neurological dysfunction .The wet and dry weight method was used to detect the water content of brain tissue .The Nissl staining method was used to observe the pathological changes in the brain tissue , and count the number of intact neurons .The methods of immunohistochemistry and western blot were used to detect the expression level of TLR 4 and IL-6.Result Compared with sham operation group, the water content of brain in the model group was obviously higher (P<0.05), and the expression level of TLR4 and IL-6 around the hematoma increased (P<0.05), and accompany with severe neurological damage .Af-ter the treatment of simvastatin , both the expression level of TLR 4 and IL-6 around the hematoma and the water content of brain tissue decreased , accompanying with the up-regulation of the score of the neurological dysfunction .Conclusion Ac-tivated TLR4 after cerebral hemorrhage may participate the secondary inflammatory reaction and the pathological process of brain edema around the hematoma after cerebral hemorrhage via the activation of IL -6.Simvastatin may exert the protective effect of brain tissue via the improvement of the inflammatory response and the degree of brain edema mediated by TLR 4.