Effect and mechanism of phosphonoformic acid onβ-glycerophosphate-incudced calcification in rat vascular smooth muscle cells
HU Chun-yan
XU Jin-sheng
SHI Bo
ZHANG Jun-xia
BAI Ya-ling
CUI Li-wen
ZHANG Hui-ran
ZHANG Sheng-lei
Abstract：Objective To explore the effect and mechanism of phosphonoformic acid ( PFA) on β-glycerophosphate-incudced calcification in rat vascular smooth muscle cells (VSMCs).Methods Primary VSMCs obtained from rat thoracic aorta in vitro were cultured .After being identified with morphological and immunocytochemistry , VSMCs were added with 10 mmol/Lβ-glycerophosphate (HP group) and 10 mmol/L HP +1 mmol/L PFA (PFA group), meanwhile, the control group was set .RT-PCR and Western blot were used to detect the expression of mRNA and protein of core binding factor α1 (Cbfα1) at different time points, respectively.Results Compared with the control group, the cell calcification in the HP group and PFA group was increased (all P<0.05), while compared with HP group, the calcium deposition was signifi-cantly reduced in the PFA group(P<0.05).In the HP group, the expression of mRNA and protein of Cbfα1 were in a“peak-valley-peak” trend (P<0.05), and the first peak was higher than the second one (P<0.05).In the PFA group, the second peak of Cbfα1 mRNA moved forward, which reached the top at 6h and 1d respectively(all P<0.05).Unlike the expression of mRNA, the protein of Cbfα1 reached the top at 12h and 4d, respectively(P<0.05), and the second peak was lower than the first one(P<0.05).Conclusion PFA inhibitsβ-glycerophosphate-incudced VSMCs calcification possibly by affecting the hyperphosphate-induced phenotypic transdifferentiation of VSMCs .
Keywords：vascular smooth muscle cellsphosphonoformic acidβ-glycerophosphatecore binding factor α1
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 22-25 )