Metabonomic analysis and biomarker screening of serum for polycystic ovary syndrome patients with phlegm-dampness syndrome

LI Xiu-yang; ZHANG Ning; REN Jian; XU Wan-li; LI Yu-qiu; LIU Jia-yi

doi:10.3969/j.issn.1002-266X.2015.37.005

Abstract：Objective To study the differences in the serum metabolites for polycystic ovary syndrome ( PCOS) be-tween patients with phlegm-dampness syndrome and healthy people with the application of metabonomics based on nuclear magnetic resonance ( NMR) , and to investigate the biomarkers associated with phlegm-dampness syndrome of PCOS as well as the mechanism .Methods After the preparation of clinical serum samples collected from PCOS patients with phlegm-dampness syndrome ( phlegm-dampness syndrome group , n=20 ) and the healthy people ( control group , n=20 ) , we pre-processed the hydrogen spectrum for nuclear magnetic resonance , then using SIMCA-P+software for pattern recognition of multivariate analysis .After the data were analyzed by the principal component analysis ( PCA ) and partial least squares discriminant analysis ( PLS-DA ) , then they were amended by the orthogonal partial least squares discriminant analysis ( OPLS-DA) .According to the analysis of different metabolic components , the biomarkers of phlegm-dampness syndrome of PCOS were finally screened and identified .Results There were significant differences in the serum metabolites between the phlegm-dampness syndrome group and the control group .The levels of 1-methyl histidine, citrate, ethanol, glycerol phosphate choline, low density lipoprotein L1 and lipid L6 were lower in the phlegm-dampness syndrome group as compared with those of the control group, and statistically significant difference was found (all P<0.05).And the levels of acetoac-etate, very low density lipoprotein L5, lipid L7, lactate, lysine, n-acetyl glycoprotein and threonine were higher in the phlegm-dampness syndrome group as compared with those of the control group , and statistically significant difference was found between the two groups (all P<0.05).Conclusions These 13 metabolites could be the potential biomarkers for P-COS patients with phlegm-dampness syndrome .The change mechanism of serum metabolites in PCOS patients was closely related with the tricarboxylic acid cycle , glucose metabolism , lipid metabolism and protein metabolism , which mainly mani-fested the disorders of tricarboxylic acid cycle , slowed glucose metabolism and increased lipid and protein metabolism .