Effect of glutamine on Akt activity in small intestine mucosa of rats with obstructive jaundice

SHANG Hai-tao; LI Zhong-lian; ZHANG Xi-bo

doi:10.3969/j.issn.1002-266X.2015.36.007

Abstract：Objective To observe the activity changes of protein kinase B ( Akt) in the small intestine of rats with ob-structive jaundice, and to investigate the mechanism of glutamine in intestinal protection.Methods Sixty-four SD rats were randomly divided into four groups, 16 rats in each group.The obstructive jaundice models were made in the groups A, B and C.After freeing the upper common bile duct and then we closed the abdomen of rats in the group D.One day after modeling, rats in the groups B and C were given the same amount of heat enteral nutrition by gavage, and at the same time, the glycine and glutamine 1 g/( kg· d) was added.Rats in the groups A and D were given the same volume of normal saline by gavage. Eight rats were sacrificed at day 3 and 7 after surgery.Western blotting was used to detect the small intestine tissue’s total protein of Akt and p-Akt, RT-PCR method was used to detect Akt mRNA of intestine tissue.Results Compare with group D, the Akt mRNA and p-Akt protein was increased at day 3 and 7 after surgery in the groups A, B and C, and group C was higher than groups A and B (all P<0.05).No significant difference was found between group A and group B (all P>0.05).No sig-nificant difference was found in Akt total protein of small intestine among these groups (all P>0.05).Conclusion The gluta-mine protects the epithelial cells of small intestine by promoting the activation of Akt signaling pathway.