K-ras gene mutations in colorectal carcinoma and its clinical significance

LIU Shan; WANG Dan; LU Fu-yu; CHENG Bo; SHI Jian-ling; DUAN Lian; SUN Suo-zhu

doi:10.3969/j.issn.1002-266X.2015.36.001

Abstract：Objective To observe the K-ras gene mutations in the colorectal carcinoma tissues and to provide basis for the individualized treatment of colorectal cancer in clinical.Methods A total of 156 cases of colorectal carcinoma samples ( observation group) and 20 cases of normal tissue samples ( the control group) were collected.Genomic DNA was extrac-ted and K-ras gene mutations were analyzed by real-time fluorescence quantitative polymerase chain reaction.Then, we an-alyzed the K-ras gene mutation rate.Meanwhile, the 12th and 13th codon mutations in colorectal cancer and the relation-ships with clinicopathologic features were analyzed.Results The K-ras gene mutations were not found in the control group, and K-ras gene mutation rate in the observation group was 40.4% (63/156).Significant difference was found in the mutation rate between these two groups (P<0.05).The K-ras gene mutation rate was not related with sex, tumor loca-tion, tumor grade, pathological stage, lymph node and lymph node metastasis (all P>0.05), but was related with age (P<0.05).The mutation rate at codon 12 and codon 13 of K-ras gene was 76.2%(48/63) and 27.0%(17/63), respec-tively in the observation group, and significant difference was found (P<0.05).The mutation at codon 12 and codon 13 of K-ras gene was related with tumor differentiated degree (P<0.05), but not related with sex, age, tumor location, patho-logical stage, lymph node and lymph node metastasis (all P>0.05).Conclusions K-ras gene mutation rate in the color-ectal carcinoma tissues is 40.4%, and the most common type is the codon 12 ( GGT-GAT) mutation.The detection of K-ras gene mutation helps to provide basis for the individualized treatment of colorectal cancer in clinical.