Influence of BMSCs-derived exosome on proliferation and invasion of mouse breast cancer cells 4 T1 and the mechanism
WANG Dan-dan
CHEN Jian-zhong
KANG Chun-yan
Abstract：Objective To observe the influence of bone marrow mesenchymal stem cells ( BMSCs)-derived exosome on the proliferation and invasion of mouse breast cancer cells 4T1 and to investigate the mechanism.Methods The mouse breast cancer cells 4T1 were randomly divided into three groups:4T1+vehicle group, 4T1+exosome group and 4T1+exosome+Y294002 (an inhibitor of PI3K/Akt signaling pathway) group.4T1+vehicle group was added with 400μL se-rum-free medium, 4T1+exosome group was added with 400 μL serum-free medium containing 400 μg/mL exosome and 4T1+exosome+Y294002 group was added with 400 μL serum-free medium containing 400 μg/mL exosome and 5 μg/mL Y294002.We used the MTT, cell scratch test and Western blotting to examine the effect of BMSCs-derived exosome on the proliferation, migration and invasion abilities as well as the PI3K/Akt signaling pathway of 4T1 cells.Results The in-hibition rates of cell proliferation of 4T1+vehicle group, 4T1+exosome group and 4T1+exosome+Y294002 group were 0.713%±0.05%, 0.401%±0.03%and 0.459%±0.80%, respectively.Significant difference was found between 4T1+exosome group and 4T1+vehicle group, 4T1+exosome+Y294002 group (all P<0.05).The cell migration distance of 4T1+vehicle group, 4T1+exosome group and 4T1+exosome+Y294002 group was (388.0 ±36.1), (295.0 ± 34.2) and (275.0 ±63.5) μm, respectively.Significant difference was found between 4T1+exosome group and 4T1+vehicle group, 4T1+exosome+Y294002 group (all P<0.05).The OD values of p-AKT and β-catenin of 4T1+exo-some group were 0.3 ±0.11 and 0.3 ±0.08, they were 1.1 ±0.41 and 0.7 ±0.08 in 4T1+vehicle group, 0.4 ±0.13 and 0.3 ±0.07 in the 4T1+exosome+Y294002 group.Significant difference was found between 4T1+exosome group and 4T1+vehicle group, 4T1+exosome+Y294002 group (all P<0.05).Conclusion BMSCs-derived exosome can promote the proliferation, migration and invasion abilities of mouse breast cancer cells 4T1, which may be related to the up-regulation of PI3K/Akt signaling pathway.
Keywords：bone marrow mesenchymal stem cellsexosomesbreast neoplasmsbreast carcinomaPI3K/Akt signa-ling pathway
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 12-15 )