Effect of rapamycin and ciclosporin on proliferation of human periheral blood CD4+CD2+5 regulatory T cells
ZHAO Bing-qing
ZHU Zhi-jun
WANG Yong-lei
Abstract：Objective To observe the influence of rapamycin and ciclosporin on the proliferation of human periheral blood CD4+CD2+5 regulatory T cells.Methods The CD4+CD2+5 regulatory T cells ( Treg cells) from human peripheral blood were isolated by immunomagnetic bead separation, the purity of CD4+CD2+5 Treg was measured by flow cytometry.We divid-ed the CD4+CD2+5 Treg into the groups A, B and C, which were added with 100 nmol/L rapamycin, 410 nmol/L cyclospo-rine and the blank culture medium.After 7 days′culture, we calculated the percentage of primary Treg cells of different groups by flow cytometry.Results The percentage of primary Treg cells in the groups A, B and C were 8.8%±3.2%, 48.9%±7.0%and 33.2%±4.3%, respectively.Significant difference was found when we compared groups A and B with group C, respectively ( all P <0.05).Conclusion Rapamycin can significantly promote the proliferation of CD4+CD2+5 Treg in vitro, and it promotes the formation of immune tolerance in patients after transplantation;but cyclosporine inhibits the proliferation of CD4+CD2+5 Treg and hampers the progression.
Keywords：regulatory T cellsrapamycinciclosporinimmune tolerancecell culture
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 19-21 )