Effects of rifampicin on adhesion ability and biofilm formation of mycobacterium tuberculosis onto interfaces of different implant materials

ZHAO Zhi-hui; WANG Yong-qing; XIONG Qing-guang; BI Hong-bin; SUN Jin-bao; LI Yi

doi:10.3969/j.issn.1002-266X.2015.33.007

Abstract：Objective To investigate the effects of rifampicin on the adhesion ability and biofilm formation of myco-bacterium tuberculosis ( MTB) in vitro.Methods We each selected two samples of cobalt chromium molybdenum alloy, titanium alloy and polyethylene and put them into 3 centrifuge tubes, respectively.Then, 8 mL of Middlebrook7H9 culture medium and 2 mL initial concentration of 1010 cfu/mL of reinoculation of MTB suspension liquid were mixed in centrifuge tubes and cultured under the constant temperature (37 ℃) for 30 days.We randomly took out one sample from each cen-trifuge tube and used scanning electron microscope for observation after fixing, drying and spraying gold on it.Then, we compared the bioadhesion ability of MTB on different implant materials and at the same time we observed interface biofilm formation.After that, adding 2 mL concentration of 1μg/mL of rifampicin solution into each centrifuge tube.Thirty days of culturing under the same condition was performed and we took out the sample.After the same procedure with former sam-ples they were observed by scanning electron microscope, we gained a better understanding of the effect of rifampicin on the MTB adhesion force and the damage to MTB biofilm.Results The adhesion ability to polyethylene was higher than those of titanium alloy and cobalt chromium molybdenum alloy, and the adhesion ability with cobalt chromium molybdenum alloy was higher than that of titanium alloy.No biofilm forms on the interface of cobalt chromium molybdenum alloy and titanium alloy while it was available on the interface of polyethylene.After the rifampicin intervention, the number of colonies on the interface of implant reduced significantly, and the biofilm on the interface of polyethylene was destroyed to some extent. Conclusion Rifampicin can inhibit adhesion ability and biofilm formation of MTB.