Influence of Allicin on cell cycle and apoptosis of human cervical carcinoma HeLa cells
LIU Ling
NIE Dan
ZOU Qian
ZHAN Ping
REN Qian-chuan
CHEN Jian-qiong
MAO Xi-guang
Abstract：Objective To investigate the influence of allicin on the cell cycle progression and apoptosis of human cer-vical carcinoma HeLa cells.Methods The HeLa cells cultured in vitro were treated with different concentrations of allicin (2.5, 5, 10, 20, 40, 80 and 120μg/mL) for 24 h, 48 h and 72 h.Then, the morphologic changes were observed by in-verted phase contrast microscope.WST-8 was used to measure the proliferative inhibition rate of allicin on HeLa cells, and the cell cycle progression and apoptosis rate were detected by flow cytometry.Results The morphological changes of ap-optosis was observed by inverted phase contrast microscope.The proliferation of HeLa cells was inhibited after the interven-tion of Allicin, which was in a dose-and time-dependent manner(all P<0.05).With the increasing concentration of alli-cin, the apoptosis rate was increased and the proportion of G0/G1 cells was increased significantly.Conclusion Allicin can inhibit the HeLa cell proliferation, which may be related to the inducing cell apoptosis and blocking cell cycle progres-sion.
Keywords：cervical carcinomaallicinHeLa cellscell cycleapoptosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 15-17 )