Effect of sodium butyrate combined with cisplatin on cell viability and proliferation of ovarian clear cell carcinoma ES-2 cells
SU Qiang
MA Ni-na
LI Qin
YU Jing
ZHANG Chen-guang
Abstract：Objective To observe the effect of sodium butyrate combined with cisplatin on cell viability and prolifera -tion of ovarian clear cell carcinoma ES-2 cells.Methods The ovarian clear cell carcinoma ES-2 cells in the logarithmic phase were randomly divided into the observation groups and control groups .The observation groups were added 1, 2, 4 and 6 mmol/L sodium butyrate (NaB) and 5 μg/mL cisplatin.The control groups were added the same concentrations of NaB.The cell activities were detected by MMT , and the relative expression levels of c-PAPP, c-Caspase-3, ERK and p-ERK were detected by Western blotting .Results The cell viabilities of the observation groups which were treated with 1, 2, 4, 6 mmol/L NaB and 5 μg/mL cisplatin were 51%±3%, 39%±2%, 22%±2% and 11%±1%, respectively;and significant difference was found between groups (all P<0.05).The cell viabilities of the control groups were 88%± 4%, 76%±3%, 69%±2%and 54%±2%, respectively;and significant difference was found between groups ( all P<0.05).Meanwhile, significant difference was found in the cell viability between the two groups which were added with the same concentrations of NaB (all P<0.05).With the increased concentrations of NaB , the relative expression levels of c-PAPP and c-Caspase-3 in the two kinds of groups were gradually increased , and the levels of ERK and p-ERK were signifi-cantly decreased .Compared with the control groups , the relative expression levels of c-PAPP and c-Caspase-3 in the obser-vation groups which were added 1, 2, 4 and 6 mmol/L NaB and 5 μg/mL cisplatin were all increased and the levels of ERK and P-ERK were decreased in the observation groups which were added 1 and 6 mmol/L NaB and 5μg/mL cisplatin. Significant difference was found between the two kinds of groups (all P<0.05).Conclusion NaB combined with cispla-tin may decrease the cell viability of ovarian clear cell carcinoma ES-2 cells, inhibit the proliferation and promote the apop-tosis.The effect can be stronger with the higher concentrations of NaB .
Keywords：ovarian neoplasmsovarian clear cell carcinomasodium butyratecisplatincell viabilityproliferinapoptin
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )