Detection and significance of CpG unit methylation in P16 gene promoter region of esophageal squamous cell carcinoma
LIU Fang
KANG Xue
LIU Xiao-yan
ZHANG Ning
QI Cui-hua
LI Yong-jun
ZHENG Yong
CHEN Wei-gang
Abstract：Objective To detect the CpG unit methylation in the P 16 gene promoter region of esophageal squamous cell carcinoma tissues and the significance .Methods We collected 35 cases of esophageal squamous cell carcinoma sam-ples ( observation group ) and 46 cases of normal esophageal tissues ( control group ) .We used MassARRAY methylation DNA quantitative analysis technology to detect the methylation status of 19 CpG units of P16 gene promoter region , and we used Rank test to compare the CpG methylation rates of the two groups .Results The 19 CpG unit methylation rates in the P16 gene promoter region of the observation group and control group were 9.06%±7.11%and 8.48%±6.34%, respective-ly.No significant difference was found between the two groups , P>0.05.The methylation rate of P16 gene in ESCC of CpG unit 11-12 was 11.34%±8.47%in the observation group, and 7.25%±5.60%in the control group (P<0.05);and no significance was found in the methylation rate of other 18 units between the two groups ( all P>0.05 ) .Conclusion The methylation of CpG11-12 site in the P16 gene promoter region may be associated with the esophageal squamous cell carcinoma .
Keywords：esophageal squamous cell carcinomaP16 geneCpG unitmethylation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )