Effect of phosphorylated PKC-δon Dexamethasone-induced osteoblast apoptosis of rats
FU Wen-jyu
ZHANG De-zhi
WANG Guo-xi
LIU Yu-lin
Abstract：Objective To observe the effect of phosphorylated protein kinase C-δ ( PKC-δ) on Dexamethasone ( Dex)-induced osteoblast apoptosis of rats.Methods The osteoblasts of the third generation from newly born SD rats′cal-varia were divided into four groups:the control group, Dex group (1 ×10 -7mol/L Dex), PMA preconditioning group (1 × 10 -7 mol/L Dex+100 nmol/L PMA) and Rottlerin preconditioning group (1 ×10 -7 mol/L Dex+2 μmol/L Rottlerin). The cells of each group were incubated for 24 h.Then, the cell proliferation activity was measured by MTT assay, the ap-optosis was detected by AnnexinⅤ-FITC/propidium iodide ( PI)-flow cytometry ( FCM) and acridine oringe/ethidium bro-mide (AO/EB) staining, and the expression of the apoptosis-related proteins (Bcl-2, Bax and Caspase-3) and phosphoryl-ated PKC-δwas detected by Western blotting.Results Compared with the control group, the proliferation activity was re-duced, the apoptosis was increased, the expression of phosphorylated PKC-δ, Caspase-3 and Bax was increased, while the Bcl-2 expression was decreased in the Dex group, and the difference was statistically significant (all P<0.05).Compared with the Dex group, the cell proliferation activity was reduced, the apoptosis was increased, the expression of phosphoryla-ted PKC-δ, Caspase-3 and Bax was increased, while the Bcl-2 expression was decreased in the PMA preconditioning group, and the difference was statistically significant (all P<0.05).Compared with the Dex group, the cell proliferation activity was obviously higher, the apoptosis was decreased, the expression of phosphorylated PKC-δ, Caspase-3 and Bax was decreased, while the Bcl-2 expression was increased in the Rottlerin preconditioning group, and the difference was sta-tistically significant (all P<0.05).Conclusion The phosphorylated PKC-δcan promote the Dex-induced osteoblast ap-optosis of rats.
Keywords：protein kinase C-δcaspase-3B-cell lymphoma related protein xB-cell lymphoma 2osteoblastsap-optosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 20-22 )