Effects of miR-222 gene transfection on proliferation and invasion of human non-small-cell lung cancer cells

LUO Xiang-yu; LUO Wei-min; ZHANG Jun; LIN Chen-yi; GUO Jia-long

doi:10.3969/j.issn.1002-266X.2015.31.005

Abstract：Objective To investigate the effects of miR-222 gene transfection on proliferation and invasion of human non-small-cell lung cancer cells.Methods The qRT-PCR was employed to detect the expression of miR-222 in A549 and 16HBE cells.The miR-222 mimics, scramble, TIMP3-siRNA and si-control sequence were transfected into A549 cells ( experimental group 1, experimental group 2, experimental group 3 and experimental group 4), respectively.Western blotting was used to de-tect the expression of TIMP3 protein.The proliferation and invasion abilities were evaluated by MTT and Transwell invasion as-say.Results The expression of miR-222 in A549 cells and 16HBE cells was respectively 0.767 ±0.086 and 0.405 ±0.034 (P<0.01).The expression levels of TIMP3 protein in the A549 cells of the experimental group 1, experimental group 2, experi-mental group 3 and experimental group 4 were 0.416 ±0.023, 0.732 ±0.019, 0.430 ±0.020 and 0.803 ±0.025, respectively. Significant difference was found between the experimental groups 1 and 2, between the experimental groups 3 and 4 ( all P<0.05).The cell proliferation ability was increased in A549 cells after overexpressing miR-222 and silencing TIMP3 for 48 h, 72 h and 96 h (all P<0.05).After 48 h, in the A549 cells, the number of cells through the basement membrane in the experimen-tal group 1, experimental group 2, experimental group 3 and experimental group 4 were 104 ±5, 204 ±8, 99 ±4 and 188 ±7, re-spectively.Significant difference was found between the experimental groups 1 and 2, between the experimental groups 3 and 4 (all P<0.05).Conclusion The proliferation and invasion abilities of human non-small-cell lung cancer cells transfected by miR-222 are enhanced which may be related with miR-222 targeted-regulating TIMP3.