Expression changes of sialyltransferase in peripheral blood leucocytes of patients with systemic lupus erythematosus and the possible mechanism
TU Yang
CHEN Guang-liang
CAO Shan
CHEN Xiao-xiang
Abstract：Objective To observe the expression changes of sialyltransferase ( ST8SIA) in the peripheral blood leucocytes of patients with systemic lupus erythematosus ( SLE) and to investigate the possible mechanism.Methods ST8SIA1-6 was detected by RT-PCR in the peripheral blood leucocytes in 24 SLE patients and 24 normal donors. We analyzed the miRNAs that targeting 3`untranslated region ( UTR) by microarray chip.Results The expression of ST8 SIA2 , ST8 SIA3 and ST8 SIA5 was not detected in the peripheral blood leucocytes of the two groups .The relative expression levels of ST8SIA1 mRNA, ST8SIA4 mRNA and ST8SIA6 mRNA in the observation group were 1.524 ± 0.229, 13.78 ±1.669 and 2.238 ±0.387, and they were 1.129 ±0.106, 1.087 ±0.089 and 0.550 ±0.054 in the control group.The difference in ST8 SIA4 mRNA and ST8 SIA6 mRNA of the two groups was statistically different ( all P<0.05).There were 12 down-regulated plasma miRNAs that regulated the 3`UTR of ST8SIA4 mRNA and 1 up-regulated plasma miRNA.There were 4 plasma miRNAs that made the 3`UTR of ST8SIA6 mRNA down-regulated and 4 plasma miRNAs up-regulated.Conclusion The expression levels of ST8SIA4 mRNA and ST8SIA6 mRNA in patients with SLE are increased, which may be related with the abnormal expression of plasma miRNAs that can regu-late the 3`UTR of ST8SIA4 mRNA and ST8SIA6 mRNA.
Keywords：systemic lupus erythematosusα28-sialyltransferasesmiRNApolymerase chain reactionmicroarray
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )