Inhibitory effect of Hericium erinaceus polysaccharide on mice subcutaneous transplanted sarcoma and its mechanism

doi:10.3969/j.issn.1002-266X.2015.30.003

Abstract：Objective To observe the inhibitory effect of Hericium erinaceus polysaccharide on the mice subcutaneous transplanted sarcoma , and to explore its mechanism from apoptosis perspective .Methods S180 sarcoma cells were subcu-taneously injected into 50 mice to produce the models of subcutaneous tumor transplantation .After successful modeling , the tumor bearing mice were randomly divided into five groups , 10 in each group: the low-dose group , medium-dose group , high-dose group, model group and cyclophosphamide group .The low-dose, medium-dose and high-dose groups were given hedgehog polysaccharide 25, 50 and 100 mg/kg, orally, once a day, and for 10 days.The model group was given normal saline 0.2 mL/day orally and for 10 days.Cyclophosphamide was administered with 40mg/kg by intraperitoneal injection once a day for 5 days.All the mice were killed after 10 days of treatment .We calculated the inhibition rate of each group . The apoptosis in each group was detected by TUNEL assay .Caspase-3 protein was detected by immunohistochemical meth-od.Results The tumor inhibition rates in the low-dose group, medium-dose group, high-dose group and cyclophospha-mide group were 36.36%, 57.57%, 43.93%and 74.24%.The tumor inhibition rate in the cyclophosphamide group was higher than those in the other groups , and in the medium-dose group , the tumor inhibition rate was higher than those of the low-dose group and high-dose group (all P <0.05).The number of apoptotic cells and Caspase-3 protein expression were as follows:cyclophosphamide group >medium-dose group >high-dose group >low-dose group >model group ( all P<0.05).Conclusion Hericium erinaceus polysaccharide can inhibit the growth of xenograft sarcoma , and the dose of 50 mg/kg dose is the best .This mechanism may be related to its up-regulation of Caspase-3 protein expression and thereby promoting tumor cell apoptosis .