Effect of down-regulating FoxM1 expression on biological behavior of renal cancer 786-O cells
CHENG Xin-yu
WU Xiao-rong
JIA Bo-shen
SHA Jian-jun
CHEN Yong-hui
HUANG Ji-wei
ZHANG Jin
LIU Dong-ming
HUANG Yi-ran
Abstract：Objective To observe the down-regulation of forkhead box protein M 1 ( FoxM1 ) gene expression and to investigate its effect on the biological behavior of human renal cancer cell line 786-O.Methods The shFoxM1 plasmid was constructed by RNA interference sequence and shRNA .786-O cells were divided into the shFoxM 1 group and the con-trol group which were transfected with shFoxM 1 plasmid and scramble plasmid for 48 h, respectively .The expression of FoxM1 mRNA and protein was tested by real-time PCR and Western blotting .Then we analyzed the efficiency of plate colo-ny formation in one week by using colony formation assay , and we used flow cytometry to detect the cell cycle and apoptosis of 786-O cells.The migration and invasion abilities were analyzed by Transwell assay , and the OD value in 0, 24, 48 and 72 hours was tested using MTT assay to analyze the activity of proliferation .Results Compared with the control group , the FoxM1 mRNA and protein expression was decreased , the efficiency of plate colony formation and the cell proliferation were decreased, the cells were blocked in G1 phase, cells of G2 phase and S phase reduced, the early apoptosis rate rose and the migration and invasion cells were decreased in the shFoxM 1 group (all P<0.05).Conclusions Down-regulating FoxM1 gene expression suppressed the proliferation , migration and invasion abilities of 786-O cells, blocking cells in G1 phase and promoting early apoptosis .
Keywords：forkhead box protein M 1kidney neoplasmsclear cell carcinomacell proliferationapoptosisRNA in-terference technology
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )