Effect of trimetazidine hydrochloride preconditioning on rats with acute renal ischemia-reperfusion injury

DU Yi; QIAN Bai-yin; WEI Yu-ting; LI Su-xia; LI Mi

doi:10.3969/j.issn.1002-266X.2015.29.008

Abstract：Objective To observe the effect of trimetazidine hydrochloride (TMZ) preconditioning on rats with acute re-nal ischemia-reperfusion injury (IRI).Methods Ninety rats were randomly divided into two groups:group A and group B. Rats in the group A were fed with TMZ 10 mg/kg for 5days and we established the animal models of acute renal ischemia-reperfu-sion injury (IR1), ischemia reperfusion postprocessing (IPO1) and sham operation (S1).Rats in the group B were fed with normal saline for 5 days by gavage and we established the animal models of acute renal ischemia-reperfusion injury (IR2), ische-mia reperfusion postprocessing (IPO2) and sham operation (S2).Serum Scr, BUN, NGAL and IL-18 levels were determined at the end of 0, 6, 12, 24 and 48 h after restoring the renal blood flow.Morphology changes of renal tissues were observed under HE staining microscopy.Results There was no significant difference in all above levels at different time points between groups IR1 and IPO1.NGAL, IL18 and renal tube injury score at 6 h increased in the IR2 group as compared with those of the IR1 group, and BUN was also increased significantly at 12 h.All the levels of IR2 group were higher than those of the IR1 group at 24 h.The levels of BUN and Src in the IR1 group were higher than the IR1 group at 48 h (all P<0.05).When we compared the IPO1 and IPO2 groups, the levels of NGAL in the two groups increased in 6 h, all levels were increased at 12 h except for Scr;at 24 h, all levels except for NGAL were higher in the IPO2 group than those of the IPO1 group.At 48 h, BUN, Scr and re-nal tube injury score were higher than those of the IPO1 group.When we compared IR1 group with IPO2 group, at 6 h, the NGAL level was increased, and all levels of the IPO2 group were increased except for Scr at 12 h as compared with those of the IR1 group;at 48 h, the Scr level was higher than that of IR1 group (all P<0.05).Conclusions The preventive use of TMZ might alleviate the damage of renal function before renal IRI.