Effects of different modalities of sevoflurane conditioning on isolated rat hearts subjected to ischemia-reperfusion injury and the influence on gene expression profiles
YAO Yun-tai
GONG Zhi-yi
LI Li-bing
LI Li-huan
Abstract：Objective To compare the cardioprotective effects of sevoflurane preconditioning (SpreC), sevoflurane postconditioning ( SpostC ) and sevoflurane preconditioning plus postconditioning ( SpreC +SpostC ) on the isolated rat hearts with myocardial ischemia-reperfusion injury ( MIRI) , and the influence on the myocardial gene expression profiles. Methods After 10 min of equilibration, the isolated rat hearts were randomly divided into four groups:the control group ( CTRL group) , SpreC group, SpostC group, and SpreC +SpostC group.Hemodynamics were compared within and be-tween groups at baseline, before ischemia, at 15 min, 60 min and 120 min upon reperfusion.The left ventricular myocardi-al nicotinamide adenine dinucleotide ( NAD+) were compared after 15 min of reperfusion.The coronary arteries ( coro-nary) effluent of each group were taken at equilibrium and 120 min of reperfusion to detect the levels of lactate dehydrogen-ase ( LDH) and creatine phosphate kinase ( CK-MB) .Infarct size was determined at the end of 120 min of reperfusion. Left ventricular myocardium was collected at 15 min upon reperfusion to characterize the gene expression profiles.Results Compared with the CTRL group, hearts in the SpreC, SpostC and SpreC +SpostC groups showed significantly better functional recovery, reduced myocardial infarct size, decreased LDH and CK-MB release (all P<0.05), and the myocar-dial protective effect of the SpreC+SpostC group was better than that of SpreC or SpostC group (all P<0.05).Myocardial NAD+contents in the SpreC, SpostC, SpreC +SpostC groups were higher than that in the CTRL group (all P<0.05), and SpreC+SpostC group was higher than either SpreC or SpostC group ( all P<0.05) .Microarray genome analysis dem-onstrated that few genes were jointly regulated by SpreC, SpostC and SpreC +SpostC.Conclusions SpreC and SpostC were equally effective in protecting against MIRI.The combination of SpreC and SpostC offered additive protection.Howev-er, three sevoflurane treatment strategies had different influence on the myocardial gene expression profiles.
Keywords：reperfusion injurysevofluranemitochondrial permeability transition poremicroarrayrats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 17-19,22 )