Screening differentially expressed genes of pancreatic cancer by cDNA microarray
JIA Chang-he
LIU Zhi-liang
ZHANG Cheng-wei
Abstract：Objective To investigate the feasibility of using cDNA microarray to screen differentially expressed genes between pancreatic cancer tissues and surrounding normal pancreatic tissues.Methods Gene chip containing 27 958 genes made in Agilent company was used to detect gene expression profiles between tumor tissues and surrounding normal tissues from four patients with pancreatic cancer respectively, and we identified the differentially expressed genes of pancre-atic cancer.The expression of CD151, S100A4, TIMP-3 and NME3 was detected by sqRT-PCR.Results The analysis showed that 46 genes were identified as differentially expressed genes of pancreatic cancer, including 26 up-regulated genes and 20 down-regulated genes.The expression of CD151, S100A4, TIMP-3 and NME3 was consistent with gene chip analy-sis.Conclusion Differentially expressed genes associated with pancreatic cancer can be screened by cDNA microarray, which provides the foundation for the next step research of pancreatic carcinoma molecular markers.
Keywords：pancreatic neoplasmsgene microarraycarcinoma gene
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 10-13 )