Influence of thymic stromal lymphopoietin on differentiation of effector T cells
LIN Jing
LI Xiu-hong
LIANG Lei
WANG Yi
CHEN Hai-chao
YANG Zheng
YOU Peng-hua
Abstract：Objective To investigate the influence of thymic stromal lymphopoietin ( TSLP) produced by oxidized-low density lipoprotein ( ox-LDL)-induced human vascular smooth muscle cells in vitro on differentiation of effector T cells . Methods Human vascular smooth muscle cells, human dendritic cells ( DCs) and CD4+T cells were isolated and cul-tured.Then we divided them into 4 groups, and 5 samples in each group.The control group and the experimental group were separately simulated with PBS and ox-LDL.The supernatant was incubated with DCs and CD4+T cells.TSLP neutrali-zing antibody group and neutralizing antibody control group were added with TSLP neutralizing antibody, TSLP non-specific neutralizing antibody, then were co-cultured with DC and CD4+T cells.The levels of TSLP and Th17cytokines including IL-17, IL-22 and TNF-αwere detected by ELISA, and the flow cytometry was used to detect the proportion of Th17 cells.Re-sults Compared with the control group, the levels of TSLP, IL-17, IL-22, TNF-αand the proportion of Th17 cells were increased in the experimental group (all P<0.01).Compared with the neutralizing antibody control group, the levels of TSLP, IL-17, IL-22, TNF-αand the proportion of Th17 cells were decreased in the TSLP neutralizing antibody group ( all P<0.01).Conclusion TSLP produced by ox-LDL-induced human vascular smooth muscle cells in vitro could promote CD4+T cells differentiating into Th17 cells.
Keywords：thymic stromal lymphopoietindendritic cellsT cellsTh17cellsatherosclerosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 4-6 )