Storage conditions for plasma samples of pepsinogen and gastrin-17

YAO Li-juan; LUO Yi-qin; SUN An-yuan; BU Guo-ping; WANG Li

doi:10.3969/j.issn.1002-266X.2015.28.006

Abstract：Objective To investigate the storage conditions for plasma samples of pepsinogen ( PG) and gastrin-17 ( G-17) .Methods A total of 180 plasma samples were collected to detect PG-Ⅰ, PG-Ⅱand G-17 as the first day′s con-sequence ( the control group) by ELISA double antibody sandwich method.The remaining samples were divided into group 1 and group 2 with 90 samples in each.Each group was equally divided into the non-separated group with upper layer which was plasma after centrifugation, the separated group with only plasma after centrifugation and the stabilizer group with plas-ma and stabilizer, 30 cases in each group.Every sample in each group was packaged into 6 pipes which were separately stored at room temperature, 4℃and -20℃, and we took the samples from group 1 to detect PG-Ⅰand PG-Ⅱ, or G-17 in the group 2 at the same time for 7 days.The concentrations of PG-Ⅰ, PG-Ⅱand G-17 from non-separated, separated and stabilizer groups in the different storage temperatures at different time were compared with the consequences of the first day, and the days of P>0.05 was taken as the maintain time of stability.Results In the non-separated group, the plas-ma PG-Ⅰ, PG-Ⅱand G-17 maintained the levels for 2 days, 7 days and 1 day at room temperature;5 days, 7days and 3 days at 4 ℃;and 4 days, 3 days and 2 days at -20 ℃.In the separated group, the plasma PG-Ⅰ, PG-Ⅱ and G-17 could maintain the levels for 7 days, 6 days and 2 days at room temperature;5 days, 7days and 5 days at 4 ℃; and 5 days, 7 days and 6 days at -20℃.The plasma PG-Ⅰ, PG-Ⅱand G-17 in the stabilizer group could maintain the levels for 7 days, 7 days and 7 days at room temperature;5 days, 6 days and 4 days at 4 ℃;and 7 days, 5 days and 4 days at-20 ℃.Conclusion In 7 days of storage for clinic detection, PG and G-17 detection samples are recommended to stor-age in low temperature after centrifugation, and are recommended to preserve at room temperature if we add stabilizer.