Establishment of rat model of cathartic colon and expression changes of c-kit mRNA in gastrointestinal tissues
WANG Wen-ge
CI Miao-miao
ZHANG Jun-hong
LIU Xing
MENG Xiao-yu
Abstract：Objective To establish the rat model of cathartic colon and to observe the expression changes of c-kit mR-NA in the stomach, small intestine and colon tissues.Methods Twenty healthy male Wistar rats were randomly divided into two groups:the control group and constipated group, ten in each group.Rhein suspension was administered to induce cathartic colon in the constipated group.The control group was intragastrically treated with normal saline.During the mod-eling, we observed the shape of rat stool daily.At the end of modeling or one week after the end of intragastric administra-tion, rats were treated with activated carbon suspension, and we calculated the activated carbon transit rate to see if the modeling was successful or not.Then, Then c-kit mRNA expression in the gastric antrum, small intestine and colon tissues were detected by real-time PCR.Results The stool in the constipated group was significantly harden as compared with that of the control group.Carbon black transit rates in the constipated group and control group were 39.24%±4.28%and 61.84%±3.05%, respectively;and significant difference was found between the two groups (P<0.05).The rat model of cathartic colon was successful in the constipated group.The expression of c-kit mRNA in the gastric antrum, small intes-tine and colon tissues of the constipated group was respectively 0.61 ±0.08, 0.37 ±0.02 and 0.32 ±0.03, and they were 0.67 ±0.03, 0.90 ±0.05 and 1.01 ±0.09 in the control group.Significant difference was found in the c-kit mRNA ex-pression of the small intestine and colon tissues between the two groups (all P<0.05).Conclusion The rat model of ca-thartic colon could be successfully established with rhein, and the c-kit mRNA expression in the small intestinal and colon tissues is up-regulated which may be related with the pathogenesis of cathartic colon.
Keywords：constipationslow transit constipationcathartic coloninterstitial cells of Cajaltyrosine kinase growth factor receptorsanimal experimental model
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 4-6 )