Effects of cell starvation and TNF-αderived endothelial microvesicles on human brain microvascular endothelial cells
PAN Qun-wen
HE Cai-xia
LIU Ya-jing
ZHANG Hui-ting
WANG Yan
DAI Bing-yan
MA Xiao-tang
Abstract：Objective To investigate the effects of cell starvation and TNF-αderived endothelial microvesicles (EMVs) on the proliferation, migration and angiogenesis of human brain microvascular endothelial cells (HBMECs) in vitro.Methods HBMECs were cultured and were divided into the PBS group, starvation group and TNF-αgroup.EMVs were extracted from HB-MECs cultured in a serum deprivation (SD) medium (starving stress, sHB-MVs) or SD medium containing tumor necrosis fac-tor-(TNF-α) (apoptotic stress,αHB-MVs).The HBMECs of the starvation group and TNF-αgroup were cultured with sHB-MVs andαHB-MVs (1 ×108/mL, 10μL each hole), respectively;and the PBS group was treated with 10μL PBS.The prolif-eration of HBMECs in each group was determined by MTT, the migration distance of HBMECs was measured by inverted micro-scope and the number of angiogenesis was observed under the inverted microscope.Results The proliferation of HBMECs of the starvation group was higher than the PBS group, and TNF-αgroup was lower than that of the PBS group (all P<0.01).The migration distance of HBMECs in the starvation group was longer than that of the PBS group (P<0.05), and the TNF-αgroup was shorter than the PBS group (P<0.01).The number of angiogenesis of the starvation group was more than that of the PBS group (P<0.01),and the TNF-αgroup was less than the PBS group (all P<0.01).Conclusion The cell starvation-derived EMVs can promote the proliferation, migration and angiogenesis of HBMECs, but TNF-α-derived EMVs can inhibit the prolifer-ation, migration and angiogenesis of HBMECs.
Keywords：endothelial microvesiclesendothelial cellshungertumor necrosis factor
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 22-24 )