Sensitizing effect and mechanism of bortezomib on radiotherapy of nasopharyngeal carcinoma cells
WANG Di
HUANG Guan-hong
Abstract：Objective To explore the sensitizing effect and relative mechanism of bortezomib on radiotherapy of nasopha-ryngeal carcinoma cell line CNE1.Methods The CCK-8 assay was used to detect the inhibition proliferation of bortezomib (0、5、10、15、20、25、30、50、100 nmol/L) for CNE1 cells of 24 and 48 h, and we calculated the IC50 to choose the suitable concentra-tion, which was 20 nmol/L.Nasopharyngeal carcinoma cell line CNE1 was cultured in vitro and divided into the control group, the bortezomib group, the radiation group (8 Gy, 6 MV X-ray) and the combination group (20 nmol/L bortezomib+8 Gy X-ray).The apoptosis rate in each group was detected by flow cytometry.Radiosensitivity changes of CNE1 cells treated with borte-zomib were detected by colony formation assay.Western blotting was used to detect the protein expression of Rad51,γ-H2AX and PARP.Results Bortezomib inhibited the growth of CNE1 cells and it was in a dose-and time-dependent manner.The ap-optosis rate of the combination group was significantly increased .Bortezomib enhanced the radiosensitivity of nasopharyngeal car-cinoma, and the sensitizing enhancement ratio (SER) was 1.57.The expression levels of cleaved-PARP and γ-H2AX were in-creased in the combination group as compared with those of the other three groups , and the Rad51 was decreased.Conclusion Bortezomib enhances the radiosensitivity of nasopharyngeal carcinoma cell line CNE1, whose mechanism may be related with in-ducing apoptosis and inhibiting the repairing of radiation-induced DNA injuries.
Keywords：nasopharyngeal neoplasmsradiosensitizationDNA damagebortezomibapoptosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 23-25 )