Research of the effect of HMGA2 on biological behavior of cholangiocarcinoma cell RBE
XIU Peng
WANG Gui-yang
XIN Cheng
GAO Jian-xin
LI Ke-wei
Abstract：Objective To observe the effect of HMGA2 on biological behavior of the cholangiocarcinoma cell of RBE. Methods The cholangiocarcinoma cell of RBE cultured by routine method were divided into observation group and control group, each group had six holes.HMGA2 siRNA and NC siRNA were added to observation group and control group.The proliferation rate of the two groups were determined by CCK8 assay.Cell cycle distribution and cell apoptosis rate were de-tectedbyflowcytometry.Migrateabilityofthetwogroupsweredetectedbycellmigrationexperiment.Results ①Cell proliferation rate:The cell proliferation rate of observation group and control group were 0.30 ±0.01, 0.49 ±0.03, obser-vation group was lower than the control group, P<0.05.② Apoptosis rate: The early apoptosis rate of observation group and control group were 18.71%±0.61%, 11.90%±1.01%, total apoptosis rate were 25.73%±0.19%, 18.66%± 0.97%, apoptosis rate of observation group was higher than that of control group, P<0.05.③Cell migration ability:The number of the cells across the membrane in observation group and control group were 15.0 ±0.6, 15.0 ±1.5, observation group was lower than the control group, P<0.05.④Cell cycle distribution:The cells in S phase of the observation group and control group were 35.34%±1.95%, 23.82%±0.95%, observation group was higher than the control group, P<0.05.Conclusions HMGA2 siRNA can inhibit the cell proliferation and migration, it can also induce cell apoptosis and block cell cycle in S phase in the cholangiocarcinoma cell of RBE.
Keywords：CholangiocarcinomaHMGA2 siRNAcell proliferationcell apoptosiscell migrationcell cycle
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 27-29 )