Expression changes of miR-92a in colon cancer tissues and cells and its significance

YANG Chen-hao; TAN Zhi-jun

doi:10.3969/j.issn.1002-266X.2015.22.006

Abstract：Objective To observe the expression changes in levels of miR-92a in the colon cancer tissues and cells andtoexploreitsclinicalsignificance.Methods ①TherelativeexpressionlevelsofmiR-92ainthetissuesof75pa-tients with stagesⅡwith high risk factors or stagesⅢcolon cancer was detected by real-time q-PCR and was calculated by using the equation 2 -ΔΔCt , and its association with clinicopathological parameter was evaluated.②HT-29 cells were plated onto 96-well plates and were divided into the experimental group, negative control group, liposome group and control group, each group set up three holes.The experimental group was transfected with miR-92a inhibitor, the negative control group was transfected with miR-92a negative control oligonucleotide, and liposome group was transfected liposome.We de-tected the expression changes of miR-92a to evaluated the effects of transfection.Then the cells were treated with 50μg/mL oxaliplatin for 24 h, and at different time points (24, 48 and72 h), the culture medium was removed and replaced with culture medium containing 10μL CCK-8 solution.After incubation at 37℃for 2 h, the absorbance at 450 nm on each well was measured by an enzyme immunoassay analyzer.After the transfection for 48 h and being treated by oxaliplatin for 24 h, the apoptosis rate was detected and measured by TUNEL.Results The expression of miR-92a was significantly higher than that of the normal adjacent mucosa.The expression of miR-92a was not related with gender, age, tumor location, depth of invasion, lymph node metastasis, cancer embolus or tissue differentiation ( all P>0.05) , but was related with lo-cal recurrence and distant metastases (P<0.05).The experimental groups'A value of HT-29 were lower and the apoptosis rate was higher as compared with that of the other three group at different time points (24,48 and 72 h) after transfection (all P<0.05).Conclusions The expression of miR-92a in the colon cancer tissues is increased.The relative expression of miR-92a in the colon cancer tissues is related with local recurrence and distant metastases.Suppressing the expression of miR-92a can increase the chemosensitivity of colon cancer cells.