Effect of SIRT1-shRNA lentiviral expression vector transfection on biological behavior of non-small-cell lung cancer A549 cell line
SUN Shuo-wen
ZHU Zhi-yan
DU Wei
WANG Hao
LIU Zhe
Abstract：Objective To investigate effect of SIRT1-shRNA lentiviral expression vector transfection on the cell adhe-sion, migration and the expression of E-cadherin and β-catenin in non-small-cell lung cancer ( NSCLC) A549 cell line. Methods The SIRT1-shRNA lentiviral expression vector was constructed and we used it to transfect the A549 cells.The cells infected with 277 empty vector were used as the control group and the cells infected with 277-iSIRT1 were used as the observation group.The SIRT1 mRNA expression in the two groups was detected by RT-PCR.Cell adhesion status of A549 cells in the two groups was detected by cell adhesion assay, cell migration was detected by wound healing assay, and the protein and mRNA expression levels of E-cadherin andβ-catenin were detected by RT-PCR and Western blotting, respec-tively.Results the SIRT1 mRNA of A549 cells in the observation group and control group were respectively 0.48 ±0.26 and 1.00 ±0.00 (P<0.05), the adhesive cells of the observation group and control group were 196.6 ±9.8 and 125.6 ±9.8, respectively (P<0.05), the relative migration rates of the observation group and control group were 0.65 ±0.02 and 1.00 ±0.00, respectively (P<0.05).and the E-cadherin expression levels of the observation group and control group were 1.27 ±0.16 and 1.00 ±0.00 (P<0.05), andβ-catenin expression levels were 1.24 ±0.13 and 1.00 ±0.00 (P<0.05).Conclusion SIRT1-shRNA lentiviral expression vector may significantly down-regulate the expression of SIRT1 in A549 cells, increase the expression levels of E-cadherin andβ-catenin mRNA and protein, enhance the capacity of cell ad-hesion and reduce the cell migration ability.
Keywords：non-small-cell lung cancer ( NSCLC)SIRT1-shRNA lentiviral expression vectorSIRT1 geneE-cad-herin proteinβ-catenin proteincell adhesioncell migration
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 12-15 )