Effect of carvedilol on expression of leptin and leptin receptor in liver fibrosis rat models
LIU Na
MU Hua
LIANG Chuan-dong
WANG Yu-zhen
ZHENG Ji-min
ZHANG Jian
Abstract：Objective To investigate the effect and mechanism of carvedilol on inhibition of liver fibrosis.Methods Thirty-six male SD rats were randomly divided into groups:the control group (n=6), model group (n=10), the carve-diloltreatment group 1 (n =10) and the carvedilol treatment group 2 (n =10).Liver fibrosis rat models were establishedthrough common bile duct ligation.After the models were accomplished for 48 hours, the rats of treatment groups 1 and 2were gavaged with carvedilol 1 mg/kg twice a day for 2 weeks and 4 weeks.The rats in the control group and model groupwere gavaged with equal quantity distilled water at the same time.After the experiments, liver tissue sections were preparedfrom left lobe of liver, routine HE staining and Masson trichrome staining were used to observe the liver fibrosis in eachgroup.The expression of leptin and soluble leptin receptor (sOB-R) in the liver tissues of each group was detected byWestern blotting, and the mRNA expression of leptin and leptin receptor was detected with real-time Q-PCR.Results HEand Masson trichrome showed, cholestatic liver fibrosis rat model was successfully established.The degree of liver fibrosisaggravated gradually as the molding time extension.The liver fibrosis in the carvedilol treatment group 1 and group 2 wasdecreased significantly as compared with that of the model group, and the pathological stage of liver fibrosis reduced significantlyas compared with that of the model group (P <0.05).No statistically significant difference was found between treatmentgroup 1 and treatment group 2 (P >0.05).The protein and mRNA expression of leptin and sOB-R in the rat liver tissuesof the model group was significantly higher than that of the control group (all P <0.05).The protein and mRNA expressionof leptin and sOB-R in the rat liver tissues of the carvedilol treatment groups 1 and 2 was significantly decreased ascompared with that of the model group (all P <0.05), but the differences between treatment group 1 and treatment group2 was not statistically significant (P >0.05).Conclusion Carvedilol can inhibit the process of liver fibrosis, whosemechanism may be the down-regulation of the expression of leptin and sOB-R in the liver tissues.
Keywords：hepatic fibrosisratscarvedilolleptinsoluble leptin receptor
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 9-11 )