Influence of EGCG on SHP1 expression in human glioblastoma U251 cell line
WU Bing-shan
CHENG Hong-wei
WANG Wei-hong
LI Zhi-fan
XIE Yu-huan
HE Hao-yuan
YANG Lu-lu
DONG Hao
Abstract：Objective To observe the SHP1 expression changes and methylation in the promoter region of human glio-blastoma U251 cell line after the treatment of Epigallocatechin Gallate(EGCG), and to investigate the effect and mecha-nism of EGCG on the cell proliferation of U251 cell line.Methods U251 cells in the logarithmic phase were treated by different concentrations of EGCG.The cell proliferation was detected by CCK-8.The SHP1 gene and protein expression of U251 cells was evaluated by RT-PCR or Western blotting.MSP was used to testify methylation in the promoter region of SHP1 after EGCG treatment.Results After the treatment of different concentrations of EGCG, the proliferation of U251 cell was inhibited, and the inhibitory effect was in a concentration-dependent manner.The SHP1 mRNA and protein ex-pression was elevated in U251 cells, and the promoter methylation of SHP1 was reversed from hypermethylation to hypom-ethylation.Conclusions EGCG may increase SHP1 expression by causing the methylation in the promoter region of SHP1 of the human glioblastoma U251 cell line, and thus inhibit the proliferation of U251 cell.
Keywords：glioblastomaEpigallocatechin Gallatecell proliferationprotein tyrosine phosphataseDNA methylation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 20-22,25 )