Chemotaxis of mesenchymal stem cells in human umbilical cord tissues on tumor in mice
FANG Jing-chao
ZHANG Xiao-long
ZHANG Qing
CAO Lei
JIANG Ai-na
LI Qian
Abstract：Objective To observe the tropism of human umbilical cord-derived mesenchymal stem cells ( MSCs) for malignant lymphoma of B department and liver carcinoma by using in vivo bioluminescent imaging system ( IVIS) .Meth-ods The lentivirus expression vector pLenti-fLuc was constructed by restriction enzyme cleavage and linkage.Human um-bilical cord Wharton's Jelly-derived MSCs ( WJ-MSCs) were labeled with firefly luciferase ( fLuc) by stable transfection with lentivirus which had been packaged in 293T cells.To demonstrate the tumor tropism of WJ-MSCs, MSC.fLuc were in-jected intravenously into NOD/SCID mice bearing BJAB lymphoma or BALB/c nude mice which burdened hepatocellular carcinoma in situ with HepG2 cells, and the migration in vivo was monitored by IVIS.Results The lentivirus expression vector pLenti-fLuc was successfully constructed, and the expression of fLuc was stable in WJ-MSCs by transfection with lentivirus.The subcutaneous BJAB lymphoma xenograft model was successfully established in NOD/SCID mice, and MSC. fLuc migrated to tumor site after 24 hours by intravenous injection.The HepG2 orthotopic hepatocarcima model was success-fully established in BALB/c nude mice.When it was injected intravenously, MSC.fLuc's tropism for liver was found 48 hours later.Conclusion WJ-MSCs have the capacity of tumor tropism for lymphoma and hepatocarcima, and it provides basis for tumor-targeting therapy which uses MSCs as the vector.
Keywords：lymphomaliver neoplasmsmesenchymal stem cellsbioluminescence imagingchemotaxismice
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-3,9 )