Relationship between white blood cell mitochondrial DNA content in first trimester of pregnancy and prevalence of gestational diabetes mellitus
CHEN Yi-mei
ZHANG Tong
LI Wan-gen
CHEN Yong-xia
NIU Jian-min
WANG Fang
LI Zi-hao
Abstract：Objective To investigate whether white blood cell mitochondrial DNA ( mtDNA) content in the first tri-mester of pregnancy is associated with gestational diabetes mellitus ( GDM) .Methods Data and blood samples were col-lected from 206 females who were diagnosed as pregnancy within 12 weeks.The mtDNA quantification using nuclear DNA ( nDNA) as a reference was analyzed by real-time quantitative polymerase chain reaction ( PCR) .According to the median value of the mtDNA/nDNA ratio, the subjects were divided into two groups: lower mtDNA content group (n=103) and higher mtDNA content group (n=103).The fasting glucose and fasting insulin were measured and homeostasis model as-sessment of IR (HOMA-IR) was calculated.Oral glucose tolerance test (OGTT) was taken between week 24 and 28 of gestation, and the prevalence of GDM in the two groups was observed .Results In the first trimester of pregnancy from week 24 to week 28, the prevalence of GDM in the lower mtDNA content group and higher mtDNA content group was 10. 7%and 6.8%, respectively (P>0.05).There was no statistical significance in fasting glucose , fasting insulin and HO-MA-IR between thess two groups (all P>0.05).Conclusion The content of white blood cell mtDNA in the first trimester of pregnancy is not a risk factor for GDM .
Keywords：pregnancy complicationsmitochondrial DNA contentgestational diabetes mellitusfirst trimester of pregnancy
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 22-24 )