Relationships of mitochondrial tRNAleu mutations with tumorigenesis and p53 mutation
WANG Ben-shi
QIAO Lu-xin
ZHANG Yu-lin
FENG Xiao-kun
CHEN De-xi
GUO Hong-liang
Abstract：Objective To investigate the relationships of T→C mutation of 3264 site in tRNAleu region of mitochon-drial DNA (mtDNA) with tumorigenesis and p53 mutation.Methods The whole DNA was extracted from carcinoma and adjacent tissues of 28 patients with liver carcinoma and 35 patients with colon cancer .Then tRNAleu region of mtDNA and p53 gene were amplified by PCR and sequenced .Results No T→C mutation was found in 3264 site, but 16 cases of mu-tations in the other sites were discovered in the carcinoma tissues .No T→C mutation was found in 3264 site, but 3 cases of mutations were discovered in the adjacent tissues .Seventeen cases of p 53 gene mutation were found in the carcinoma tis-sues;9 cases of p53 mutation were found in patients with colon carcinoma , including 6 cases of tRNAleu mutation and 3 cases of non-tRNAleu mutation (P<0.05);and 8 cases of p53 mutation were found in patients with liver cancer , inclu-ding 5 cases of tRNAleu mutation and 3 cases of non-tRNAleu mutation (P<0.05).Conclusions The T→C mutation in 3264 site of tRNAleu region can′t be considered to have relationship with tumorigenesis .The mutations in other sites of tR-NAleu region are due to p53 mutation, so the gene loses its protection , and thus the mutation occurs .
Keywords：colonic carcinomaliver carcinomamitochondriap53sequence of tRNAleu regionmutation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 7-9 )