Application of gene chip technology for screening and identifying taxol-resistant related genes in human endometrial carcinoma cell line

LIN Feng; ZHENG Jun; ZHOU You-zhen

doi:10.3969/j.issn.1002-266X.2015.19.007

Abstract：Objective To screen and identify the acquired taxol ( TAX)-resistant related genes in human endometrial carcinoma cell line by applying gene chip technology.Methods We established the Ishikawa/taxol ( TAX)-resistant cell line by intermittent and repeated exposure to TAX of a high concentration on the human endometrial carcinoma cell line Ish-ikawa.The genes of Ishikawa/TAX and Ishikawa cells were examined by using Affymetrix Human Genome U133 Plus2.0 expression profile chip.The differentially expressed genes were classified by Gene Ontology ( GO) cluster analysis.Real-time quantitative PCR ( qRT-PCR) was performed to confirm part of the differentially expressed genes.Results Compared with Ishikawa cells, there were 110 significantly differential expression genes in Ishikawa/TAX cells (ratio>3), of which the up-regulated and down-regulated genes were 87 and 23, respectively.The classification of GO involved the cell signal transduction and regulation, cell adhesion molecule, cell metabolism and transcription regulation, etc.S100A12 was up-regulated significantly, followed by CYP1B1, FUBP1 and CEACAM6.TNFAIP3, CD44 and DKK1 were significantly down-regulated.The qRT-PCR showed that S100A12 and CYP1B1 was highly expressed in the drug-resistant cells, and TN-FAIP3 and CD44 was lowly expressed in the drug-resistant cells, which coincided well with the results of cDNA microarray. Conclusion Gene chip technology screens that the up-regulated genes of acquired TAX-resistant related genes in human endometrial carcinoma cell line are S100A12, CYP1B1, FUBP1 and CEACAM6, and the down-regulated genes are TN-FAIP3, CD44 and DKK1.