Comparison of tumorigenic effect between inoculating method of slicing subaxillary skin and suturing and method of adopting paracentesis trocar of human nasopharyngeal carcinoma on nude mice
WU Yuan
ZHOU Xun-zhao
ZHONG Chang-tao
YAN Kui
WEI Zheng-bo
XIE Ying
Abstract：Objective To compare the tumorigenic effect between the inoculating method of slicing the subaxillary skin and suturing and the method of adopting the paracentesis trocar of human nasopharyngeal carcinoma on the nude mice. Methods The samples of subcutaneous transplanted tumors in nude mice were established with nasopharyngeal carcinoma HONE-1 cells.Eight BALB/c nude mice were randomly divided into two groups:group A and B, 4 mice in each group. The right subaxillary skin of each mouse was subcutaneously inoculated with the tissue masses through slicing the skin, or adopting the paracentesis trocar in the two groups, respectively.The occurrence time, volume and mass of tumor in each group were compared.Results The occurrence time of tumor in each group was 3 days after being inoculated.Compared with group B, the occurrence time of dispirited mind, coarse skin, shineless hair, loss of appetite, low activity, distinct spinal column, hunched backs and other conditions were earlier in the group A.The weight of mice was decreased in both groups, the growth of tumor in the group A was faster than that of the group B, but no significant difference was found be-tween these two groups.The mass of tumor in the group A and group B was (0.85 ±0.39) g and (0.75 ±0.30) g, re-spectively;and no significant difference was found between these two groups (P>0.05).Conclusion Both the inocula-ting method of slicing the subaxillary skin and suturing and the method of adopting the paracentesis trocar of human naso-pharyngeal carcinoma on the nude mice could receive finely tumorigenic effect.
Keywords：nasopharyngeal neoplasmsanimal experimental modeltumorigenicityinoculating method of slicing and suturing the skininoculating method of adopting the paracentesis trocar
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )