Effects of chronic Aspergillus fumigatus exposure on bronchial hyperresponsiveness, airway inflammation and remodeling in rats with bronchial asthma
WANG Ze
GAO Yan-yan
LIU Yong-quan
GAO Fu-sheng
Abstract：Objective To investigate the effects of chronic Aspergillus fumigatus ( A.fumigatus ) exposure on the bronchial hyperresponsiveness , airway inflammation and remodeling in rats with bronchial asthma .Methods Sixty-four male Wistar rats were randomly divided into group A ( chronic asthma group ) , group B ( chronic asthma +Af spore inha-lation group), group C (chronic asthma +saline inhalation group), group D (OVA-sensitized and saline-challenged +saline inhalation group ) and group E ( OVA-sensitized and saline-challenged +Af spore inhalation group ) .Meanwhile , group B was then divided into groups B1, B2 and B3 (Af spore inhalation for 1 week, 3 weeks and 5 weeks) (n=8).The airway resistance ( Raw ) and change rate of R aw after administration of acetylcholine chloride were measured by using a com-puterized system.The concentration of total serum IgE and IL-5, IL-13, IFN-γ, TGF-βlevels in the bronchoalveolar lav-age fluid ( BALF) were measured by ELISA .Lung sections were made and then received hematoxylin and eosin ( HE) stai-ning, periodic acid Schiff ( PAS) staining and Masson trichrome staining .The images were analyzed morphometrically to e-valuate the airway epithelial detachment , goblet cell hyperplasia and subepithelial fibrosis in rats of the 7 groups .Results The change rate of R aw after administration of acetylcholine chloride , the IL-5, IL-13, TGF-βlevels, the concentration of total serum IgE, the inflammatory cell infiltration, the eosinophils, the airway epithelial detachment length/epithelial diam-eter, the goblet cell hyperplasia and subepithelial collagen deposition were all higher than those in the groups of A , C, D and E(P<0.05 or P<0.01).Conclusions Chronic Aspergillus fumigatus (A.fumigatus) exposure aggravates the bronchial hyperresponsiveness , airway inflammation and the degree of airway remodeling in rats with bronchial asthma .
Keywords：bronchial asthmaAspergillus fumigatesbronchial hyperresponsivenessairway inflammationairwayremodelingrats
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 30-33 )