Monitoring effects of combined detections of ATP and T lymphocyte subsets in CD4+T lymphocytes of peripheral blood on immune function after liver transplantation
LIU Wei
LI Dai-hong
WANG Kai
LIU Chun
Abstract：Objective To investigate the monitoring effects of combined detections of ATP and T lymphocyte subsets in CD4+T lymphocytes of peripheral blood on immune function in patients after liver transplantation .Methods Eighty ca-ses of liver transplant recipients , including 30 cases of patients with stable condition after operation (stable group), 30 ca-ses of patients with infection ( infection group ) and 20 patients with acute rejection ( rejection group ) , and 30 healthy con-trols ( control group ) were selected .The concentrations of ATP in CD 4+T lymphocytes were detected by Cylex +ImmuKnow method, and the percentages of T-lymphocyte subsets were determined by flow cytometry .The changes of ATP and T lym-phocyte subsets were compared in each group and their correlations were analyzed .Results The ATP level of the infection group was significantly lower than that of the stable and control groups , and the ATP level of the rejection group was signifi-cantly higher than that of the stable and control groups (all P<0.05).Compared with the stable group, the CD3+and CD4+T lymphocyte percentage , CD4/CD8 in the peripheral blood of the rejection group were significantly higher ( all P<0.05);and the CD3+and CD4+T lymphocyte percentage , CD4/CD8 in the peripheral blood of the infection group were sig-nificantly lower (all P<0.05).In the infection group, CD3+and CD4+was positively correlated with ATP, but CD4+/CD8+had no correlation with ATP .In the rejection group , CD4+/CD8+was positively correlated with ATP , but CD3+and CD4+were not correlated with ATP .The ATP significantly increased after the cure of infection in the infection group , and the ATP significantly decreased after the cure of rejection in the injection group (P<0.05).Conclusion The combined de-tections of ATP and T lymphocyte subsets in CD 4+T lymphocytes of peripheral blood in patients after liver transplantation have important significance to the judgment of the recipients immune status , infection and induced immune tolerance .
Keywords：liver transplantationATPT-lymphocyte subsetsinfectionrejectionorgan transplantationimmune function
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 24-26 )