Protective effect of sevoflurane preprocessing combined with postprocessing on lung ischemia-reperfusion injury in rats and its mechanism
LIU Bei
TANG Dong-mei
DU Ning
XU Gui-ping
Abstract：Objective To explore the protective effect of sevoflurane preprocessing combined with postprocessing on lung ischemia-reperfusion injury ( IRI) in rats and its mechanism .Methods Fifty-four adult male SD rats were randomly divided into three groups.Rats in the sham operation group (group S) only received dissociation of the left pulmonary hi-lum without blocking .The lung ischemia-reperfusion group ( I/R group) received blocking the left pulmonary hilum for 45 minutes and reperfusion for 120 minutes for establishment of the rat model of IRI .The sevoflurane preprocessing combined with postprocessing group (SP group) received inhalation of 2.1%sevoflurane for 30 minutes prior to blocking the left pul-monary hilum, then blocking the left pulmonary hilum for 45 minutes, and inhaling 2.1%sevoflurane again for 30 minutes in the immediate release of the vascular clamp for establishment of the IRI model .Six rats were randomly sacrificed after reperfusion for 30, 60 and 120 minutes, respectively.Their lung tissues were taken for measuring the wet-to-dry weight ra-tio ( W/D ratio ) .The superoxide dismutase ( SOD ) activity was detected by colorimetry .The tumor necrosis factor-α( TNF-α) level was detected by enzyme-linked immunosorbent assay ( ELISA) .The mRNA expression of angiotensin-con-verting enzyme ( ACE) was measured by RT-PCR.The lung histopathology results were observed under the light micro-scope.Results Compared with group S , the W/D ratios, TNF-αlevels and ACE mRNA expression in the lung tissues were increased at each reperfusion time point in the I /R group and SP group (all P<0.05), and the SOD activities were decreased (all P<0.05).Compared with the I/R group, the W/D ratios, TNF-αlevels and ACE mRNA expression in the lung tissues were decreased at each reperfusion time point in the SP group (all P<0.05), and the SOD activities were elevated (all P<0.05).Under the light microscope, the lung histopathological injuries in the SP group were alleviated as compared with those of the I/R group.Conclusion Sevoflurane preprocessing combined with postprocessing may have a protective effect on lung IRI in rats by inhibiting the oxidative stress responses and inflammatory responses .
Keywords：ischemia-reperfusion injurysevofluranesuperoxide dismutasetumor necrosis factor-α
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )